商业布草的清洗与消毒概述

讨论了商业布草清洗消毒的现状,阐述了目前国内商业布草洗涤存在的问题及现在普遍使用的检测方法和标准,并分析了布草残留的主要原因,进一步探讨了商业布草洗涤领域热议的新发展、新技术。旨在让更多人了解到商业布草的现状,得到业内人士的关注,激发更多研究人员与管理人员改善商业布草洗涤消毒现存问题的欲望,进一步满足人们对高质量生活的要求。     

碳酸酐酶在功能化Fe3O4-SiO2核壳纳米颗粒上的固定及其性能表征研究

以Fe3O4纳米颗粒为核,正硅酸乙酯（TEOS）为前驱体,通过St?ber法合成了Fe3O4-SiO2核壳纳米颗粒。以γ-缩水甘油醚氧丙基三甲氧基硅烷（GPTMS）为改性剂对纳米颗粒进行改性,得到了环氧基功能化的Fe3O4-SiO2核壳纳米颗粒,随后将碳酸酐酶（carbonic anhydrase,CA）共价固定到纳米颗粒上,以实现CA固定化。采用傅立叶红外（FTIR）、透射电镜（TEM）等手段对载体材料进行表征;以对硝基苯酚乙酸酯（p-NPA）为底物,采用紫外分光光度仪测试酶活力,并对固定化酶进行了性能表征。试验结果表明,固定化酶的热稳定性、贮藏稳定性和循环使用性均优于同等条件下的游离酶,其在循环使用10次后仍保持84.2%的相对酶活力。     

Fabry Disease and the Heart: A Comprehensive Review

Fabry disease (FD) is an X-linked lysosomal storage disorder caused by mutations of the GLA gene that result in a deficiency of the enzymatic activity of α-galactosidase A and consequent accumulation of glycosphingolipids in body fluids and lysosomes of the cells throughout the body. GB3 accumulation occurs in virtually all cardiac cells (cardiomyocytes, conduction system cells, fibroblasts, and endothelial and smooth muscle vascular cells), ultimately leading to ventricular hypertrophy and fibrosis, heart failure, valve disease, angina, dysrhythmias, cardiac conduction abnormalities, and sudden death. Despite available therapies and supportive treatment, cardiac involvement carries a major prognostic impact, representing the main cause of death in FD. In the last years, knowledge has substantially evolved on the pathophysiological mechanisms leading to cardiac damage, the natural history of cardiac manifestations, the late-onset phenotypes with predominant cardiac involvement, the early markers of cardiac damage, the role of multimodality cardiac imaging on the diagnosis, management and follow-up of Fabry patients, and the cardiac efficacy of available therapies. Herein, we provide a comprehensive and integrated review on the cardiac involvement of FD, at the pathophysiological, anatomopathological, laboratory, imaging, and clinical levels, as well as on the diagnosis and management of cardiac manifestations, their supportive treatment, and the cardiac efficacy of specific therapies, such as enzyme replacement therapy and migalastat.     

Efficient Linear dsDNA Tagging Using Deoxyuridine Excision**

Site-specific strategies for exchanging segments of dsDNA are important for DNA library construction and molecular tagging. Deoxyuridine (dU) excision is an approach for generating 3’ ssDNA overhangs in gene assembly and molecular cloning procedures. Unlike approaches that use a multi-base pair motif to specify a DNA cut site, dU excision requires only a dT→dU substitution. Consequently, excision sites can be embedded in biologically active DNA sequences by placing dU substitutions at non-perturbative positions. In this work, I describe a molecular tagging method that uses dU excision to exchange a segment of a dsDNA strand with a long synthetic oligonucleotide. The core workflow of this method, called deoxyUridine eXcision-tagging (dUX-tagging), is an efficient one-pot reaction: strategically positioned dU nucleotides are excised from dsDNA to generate a 3’ overhang so that additional sequence can be appended by annealing and ligating a tagging oligonucleotide. The tagged DNA is then processed by one of two procedures to fill the 5’ overhang and remove excess tagging oligo. To facilitate its widespread use, all dUX-tagging procedures exclusively use commercially available reagents. As a result, dUX-tagging is a concise and easily implemented approach for high-efficiency linear dsDNA tagging.     

浅谈浆土路修筑技术在大型露天矿山的应用前景

为解决德兴铜矿采区运输道路雨季严重翻浆、旱季尘土飞扬的难题,通过一年多的考察调研,充分了解了浆土路的应用案例和推广动态,并全方位地了解其施工、运行和维护的整体过程,以及带来的成效。同时,对比分析浆土路应用过程中的道路参数、运输设备运行参数和黏土属性,类比分析云南集团鹤庆北衙矿业有限公司（简称“北衙金矿”）浆土路试验道路和锡林浩特国家能源胜利露天煤矿（简称“胜利露天煤矿”）FRT生物酶道路的运行效益。结果表明,德兴铜矿采用浆土路修筑技术是可行的,能大幅度降低采区道路运行和维护成本。     

地下金属矿山斜坡道浆土路修筑工艺及应用研究

斜坡道是地下金属矿山的主要运输通道,其道路质量直接影响着矿山的安全运输和经济效益。针对斜坡道混凝土浇筑路面存在的筑路成本高、养护时间长、使用寿命短及维护困难等不足,引入浆土路修筑技术,开展了地下金属矿山斜坡道浆土路筑路材料配比试验、黏土搓条和崩解试验、水洗筛分试验,并在云南卡房分公司完成了浆土路试验路段的修筑。结果表明：浆土路筑路技术能够很好地应用于地下矿山道路修筑,具有施工工艺简单、筑路成本低、施工周期短、承载能力强、抑尘防滑和低碳环保等诸多优点,为地下金属矿山道路修筑提供了工程借鉴,对于矿山降本增效、节能减排及安全高效具有重要的意义。     

表面活性剂包衣脂肪酶在有机溶剂中催化酯合成

用合成的谷氨酸二烷基酯核糖醇对来源于Candidarugosa的脂肪酶进行了包衣 ,以月桂酸与月桂醇的酯化为模型反应 ,研究了各种操作条件对包衣酶活性的影响。结果表明 ,包衣酶制备过程中的缓冲溶液的最适 pH为 6.8,谷氨酸双十二烷基酯核糖醇的包衣效果最好 ,最适反应温度为 3 0℃ ,最佳溶剂为异辛烷。在 10h内底物转化率达 94%。     

新型功能高分子材料及其应用

功能高分子是一类具有特殊用途的高分子材料 ,印迹高分子、敏感性水凝胶和固定化酶是三种较有特色的功能高分子材料。该文将对上述三种功能高分子材料以及它们在生化分离、生物催化、物质分析与检测以及药物控制释放中的应用做一介绍 ,同时也对它们的不足和发展前景进行了评述